AQA · GCSE · Combined Science

    Course version: 8464 · Version from Sept 2018

    Classification of living organisms: Due to evidence available from chemical analysis there is now a ‘three-domain system’ developed by Carl Woese. In this system organisms are divided into:

    Chemical analysis of molecules such as ribosomal RNA showed that some prokaryotes differ as much from each other as they do from eukaryotes.

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    Study notes

    From the checked teaching notes for this specification point.

    The idea

    Chemical analysis of molecules such as ribosomal RNA showed that some prokaryotes differ as much from each other as they do from eukaryotes. Carl Woese used this evidence to propose the three-domain system, which places organisms into three broad groups above kingdom level. The domains are Archaea, Bacteria and Eukaryota. Archaea and Bacteria are prokaryotic, lacking a membrane-bound nucleus, while Eukaryota contains organisms with cells that have a nucleus, including protists, fungi, plants and animals. For example, methanogenic archaea live in oxygen-free conditions and have membrane chemistry and enzymes distinct from bacteria such as Escherichia coli. The three-domain model therefore reflects biochemical evidence rather than only visible structure.

    What earns the marks

    • Chemical analysis, especially of ribosomal RNA and other molecules, provided evidence that living organisms fall into three major groups.
    • Carl Woese developed the three-domain system based on this molecular evidence.
    • The three domains are Archaea, Bacteria and Eukaryota.
    • Archaea and Bacteria are prokaryotic domains, while Eukaryota contains organisms whose cells have a membrane-bound nucleus.
    • The three-domain system is a classification model that places domains above the kingdom level.

    Common mistakes and how to put them right

    • Naming the domains as ‘animals, plants and fungi’.

      Put it right: Those are kingdoms within Eukaryota, not the three domains.

    • Confusing Archaea with Bacteria and treating them as one group.

      Put it right: Chemical evidence separates them into two distinct domains.

    • Writing that Eukaryota contains only animals and plants.

      Put it right: Eukaryota also includes protists and fungi.

    Exam tips

    • Learn the three domain names exactly: Archaea, Bacteria and Eukaryota.
    • State that the evidence came from chemical analysis, such as RNA or molecular studies, not just visible features.
    • Use the term ‘prokaryotic’ for Archaea and Bacteria, and ‘eukaryotic’ for Eukaryota, to show precise understanding.

    Try the lesson

    The start of the Classification of living organisms: Due to evidence available from chemical analysis there is now a ‘three-domain system’ developed by Carl Woese. In this system organisms are divided into: lesson, made from checked teaching notes for this specification point.

    Have a go first

    Who developed the three-domain system of classification?

    See it

    A flowchart. At the top, a box labelled 'Chemical analysis evidence' has an arrow down to a box labelled 'Carl Woese developed the three-domain system'. From that box, three arrows lead down to three boxes side by side: 'Archaea', 'Bacteria' and 'Eukaryota'. Arrows from both 'Archaea' and 'Bacteria' lead to a single box labelled 'Prokaryotic'. An arrow from 'Eukaryota' leads to a box labelled 'Cells have a membrane-bound nucleus'.
    It shows how chemical analysis evidence led Carl Woese to develop the three-domain system, with Archaea and Bacteria as prokaryotic domains and Eukaryota containing organisms with a membrane-bound nucleus.

    Key words

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    Three-domain system

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    Molecular evidence in classification

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    Prokaryotic and eukaryotic cells

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    Carl Woese and scientific models

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    See every definition
    Three-domain system
    A classification model developed by Carl Woese that places organisms into three broad groups above kingdom level.
    Molecular evidence in classification
    Evidence from chemical analysis of molecules such as ribosomal RNA showing that some prokaryotes differ as much from each other as they do from eukaryotes.
    Prokaryotic and eukaryotic cells
    Prokaryotic cells lack a membrane-bound nucleus, while eukaryotic cells have a nucleus.
    Carl Woese and scientific models
    He used evidence from chemical analysis to propose the three-domain system, a classification model reflecting biochemical evidence rather than only visible structure.

    Label the diagram

    Some boxes have lost their names. Pick the right name for each number.

    The diagram again, with 3 boxes numbered instead of named. A flowchart. At the top, a box labelled 'Chemical analysis evidence' has an arrow down to a box labelled 'Carl Woese developed the three-domain system'. From that box, three arrows lead down to three boxes side by side: 'Archaea', 'Bacteria' and 'Eukaryota'. Arrows from both 'Archaea' and 'Bacteria' lead to a single box labelled 'Prokaryotic'. An arrow from 'Eukaryota' leads to a box labelled 'Cells have a membrane-bound nucleus'.
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