AQA · A-Level · Biology

    Course version: 7402 · Version from Sept 2016

    The role of bacteria in the nitrogen cycle in sufficient detail to…

    Full specification wording

    Nutrient cycles (A-level only): The role of bacteria in the nitrogen cycle in sufficient detail to illustrate the processes of saprobiotic nutrition, ammonification, nitrification, nitrogen fixation and denitrification.

    The nitrogen cycle involves five key processes.

    Checked against the AQA 7402 specification (opens in a new tab) on .

    • Point 5 of 7
    • 4 key terms

    Study notes

    From the checked teaching notes for this specification point.

    The idea

    The nitrogen cycle involves five key processes. Saprobiotic nutrition and ammonification are performed by saprobiotic bacteria and fungi, which secrete enzymes onto dead matter and waste, releasing nitrogen as ammonia that forms ammonium ions in soil. Nitrification involves nitrifying bacteria oxidising ammonium to nitrite, then to nitrate. This requires oxygen, so it stops in waterlogged soil. Nitrogen fixation uses nitrogen-fixing bacteria (free-living or mutualistic in legume root nodules) to reduce atmospheric nitrogen gas to ammonia. Finally, denitrification occurs in anaerobic, waterlogged soils where denitrifying bacteria reduce soil nitrates into nitrogen gas, returning it to the atmosphere and reducing soil fertility.

    What earns the marks

    • Ammonification as the release of ammonia or ammonium ions from dead material, faeces and urine.
    • Nitrification as ammonium to nitrite and nitrite to nitrate by nitrifying bacteria.
    • Nitrogen fixation as the reduction of atmospheric nitrogen to ammonia.
    • Denitrification as the reduction of nitrate to nitrogen gas under anaerobic conditions.
    • Linking aeration of the soil to more nitrification and less denitrification.

    Common mistakes and how to put them right

    • Swapping nitrification and nitrogen fixation, which are different processes carried out by different bacteria.

    • Writing that nitrifying bacteria convert ammonium straight to nitrate, missing the nitrite intermediate.

    • Placing denitrification in aerobic conditions, when it occurs in waterlogged, anaerobic soil.

    Exam tips

    • Draw the cycle as a loop of ions, nitrogen gas to ammonium to nitrite to nitrate and back to nitrogen gas, and label the bacteria on each arrow.
    • Anything that waterlogs soil favours denitrification and blocks nitrification; say so explicitly when explaining a poor yield.

    Try the lesson

    The start of the Nutrient cycles (A-level only): The role of bacteria in the nitrogen cycle in sufficient detail to illustrate the processes of saprobiotic nutrition, ammonification, nitrification, nitrogen fixation and denitrification. lesson, made from checked teaching notes for this specification point.

    Have a go first

    Which process carried out by bacteria converts nitrogen gas in the atmosphere into a form that plants can absorb?

    Listen

    The nitrogen cycle involves five key processes. Saprobiotic nutrition and ammonification are performed by saprobiotic bacteria and fungi, which secrete enzymes onto dead matter and waste, releasing nitrogen as ammonia that forms ammonium ions in soil. Nitrification involves nitrifying bacteria oxidising ammonium to nitrite, then to nitrate. This requires oxygen, so it stops in waterlogged soil. Nitrogen fixation uses nitrogen-fixing bacteria (free-living or mutualistic in legume root nodules) to reduce atmospheric nitrogen gas to ammonia. Finally, denitrification occurs in anaerobic, waterlogged soils where denitrifying bacteria reduce soil nitrates into nitrogen gas, returning it to the atmosphere and reducing soil fertility. A strong answer earns marks for these points. ammonification as the release of ammonia or ammonium ions from dead material, faeces and urine. nitrification as ammonium to nitrite and nitrite to nitrate by nitrifying bacteria. nitrogen fixation as the reduction of atmospheric nitrogen to ammonia. denitrification as the reduction of nitrate to nitrogen gas under anaerobic conditions. Exam tip: Draw the cycle as a loop of ions, nitrogen gas to ammonium to nitrite to nitrate and back to nitrogen gas, and label the bacteria on each arrow.

    See it

    A flowchart running top to bottom. Dead matter, faeces and urine leads, via saprobiotic bacteria and fungi carrying out saprobiotic nutrition and ammonification, to ammonia and ammonium ions in soil. Ammonium ions lead, via nitrifying bacteria carrying out nitrification which requires oxygen, to nitrite. Nitrite leads, again via nitrifying bacteria and nitrification requiring oxygen, to nitrate. Separately, atmospheric nitrogen gas leads, via nitrogen-fixing bacteria that are free-living or mutualistic in legume root nodules carrying out nitrogen fixation, to ammonia and ammonium ions in soil. Finally, nitrate leads, via denitrifying bacteria carrying out denitrification in anaerobic, waterlogged soil, back to atmospheric nitrogen gas.
    It shows the nitrogen cycle as a loop from dead matter through ammonium, nitrite and nitrate, with nitrogen fixation feeding in and denitrification returning nitrogen gas to the atmosphere.

    Key words

    Say what each word means before you turn the card.

    ammonification

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    nitrification

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    nitrogen fixation

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    denitrification

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    See every definition
    ammonification
    Saprobiotic bacteria and fungi secrete enzymes onto dead matter and waste, releasing nitrogen as ammonia that forms ammonium ions in soil.
    nitrification
    Nitrifying bacteria oxidise ammonium to nitrite, then to nitrate, a process requiring oxygen that stops in waterlogged soil.
    nitrogen fixation
    Nitrogen-fixing bacteria, free-living or mutualistic in legume root nodules, reduce atmospheric nitrogen gas to ammonia.
    denitrification
    In anaerobic, waterlogged soils, denitrifying bacteria reduce soil nitrates into nitrogen gas, returning it to the atmosphere and reducing soil fertility.

    Label the diagram

    Some boxes have lost their names. Pick the right name for each number.

    The diagram again, with 2 boxes numbered instead of named. A flowchart running top to bottom. Dead matter, faeces and urine leads, via saprobiotic bacteria and fungi carrying out saprobiotic nutrition and ammonification, to ammonia and ammonium ions in soil. Ammonium ions lead, via nitrifying bacteria carrying out nitrification which requires oxygen, to nitrite. Nitrite leads, again via nitrifying bacteria and nitrification requiring oxygen, to nitrate. Separately, atmospheric nitrogen gas leads, via nitrogen-fixing bacteria that are free-living or mutualistic in legume root nodules carrying out nitrogen fixation, to ammonia and ammonium ions in soil. Finally, nitrate leads, via denitrifying bacteria carrying out denitrification in anaerobic, waterlogged soil, back to atmospheric nitrogen gas.
    • 1Box 1

    • 2Box 2

    0 of 2 labelled

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