AQA · A-Level · Biology

    Course version: 7402 · Version from Sept 2016

    The hydrolysis of ATP, also from the light-dependent reaction, provides…

    Full specification wording

    Photosynthesis (A-level only): The hydrolysis of ATP, also from the light-dependent reaction, provides the additional energy for this reaction.

    Reduced NADP supplies the hydrogen for the reduction of glycerate 3-phosphate, but reduction also needs energy, and that comes from ATP made in the light-dependent reaction.

    Checked against the AQA 7402 specification (opens in a new tab) on .

    • Point 3 of 7
    • 4 key terms

    Study notes

    From the checked teaching notes for this specification point.

    The idea

    Reduced NADP supplies the hydrogen for the reduction of glycerate 3-phosphate, but reduction also needs energy, and that comes from ATP made in the light-dependent reaction. ATP is hydrolysed to ADP and inorganic phosphate by ATP hydrolase, releasing energy in a small, immediately usable amount, and the inorganic phosphate released can phosphorylate other compounds and make them more reactive. Two of the three ATP molecules used in each turn of the Calvin cycle drive the conversion of GP to triose phosphate; the third is used in regenerating ribulose bisphosphate from triose phosphate, a step with an energy cost of its own. The ADP and phosphate produced return to the thylakoid membranes, where photophosphorylation converts them back to ATP, so the two halves of photosynthesis continuously recycle the same pool of nucleotide and coenzyme.

    What earns the marks

    • ATP being hydrolysed to ADP and inorganic phosphate, releasing energy.
    • That energy being used, together with reduced NADP, to convert glycerate 3-phosphate to triose phosphate.
    • ATP also being used to regenerate ribulose bisphosphate from triose phosphate.
    • ADP and phosphate returning to the light-dependent reaction to be used in photophosphorylation.
    • One mark, in a limiting factor question, for less ATP causing less GP to be reduced and less RuBP to be regenerated.

    Common mistakes and how to put them right

    • Saying ATP 'gives energy' without naming its hydrolysis to ADP and inorganic phosphate.

    • Crediting ATP with the reduction itself, when the hydrogen comes from reduced NADP.

    • Forgetting that ATP is also needed to regenerate RuBP, not only to reduce GP.

    • Describing ATP as a long-term energy store rather than an immediate energy source.

    • Writing that ATP is broken down by respiration inside the chloroplast rather than hydrolysed by ATP hydrolase.

    Exam tips

    • Pair the two requirements in one sentence: reduced NADP provides the hydrogen, hydrolysed ATP provides the energy.
    • Name both uses of ATP in the Calvin cycle whenever the question is worth two marks or more.
    • Track ADP and phosphate back to the light-dependent reaction to show that you understand the cycle is closed.

    Try the lesson

    The start of the Photosynthesis (A-level only): The hydrolysis of ATP, also from the light-dependent reaction, provides the additional energy for this reaction. lesson, made from checked teaching notes for this specification point.

    Have a go first

    In the light-independent reaction, ATP is hydrolysed to provide energy. Name the products formed when one molecule of ATP is hydrolysed.

    Listen

    Reduced NADP supplies the hydrogen for the reduction of glycerate 3-phosphate, but reduction also needs energy, and that comes from ATP made in the light-dependent reaction. ATP is hydrolysed to ADP and inorganic phosphate by ATP hydrolase, releasing energy in a small, immediately usable amount, and the inorganic phosphate released can phosphorylate other compounds and make them more reactive. Two of the three ATP molecules used in each turn of the Calvin cycle drive the conversion of GP to triose phosphate; the third is used in regenerating ribulose bisphosphate from triose phosphate, a step with an energy cost of its own. The ADP and phosphate produced return to the thylakoid membranes, where photophosphorylation converts them back to ATP, so the two halves of photosynthesis continuously recycle the same pool of nucleotide and coenzyme. A strong answer earns marks for these points. ATP being hydrolysed to ADP and inorganic phosphate, releasing energy. that energy being used, together with reduced NADP, to convert glycerate 3-phosphate to triose phosphate. ATP also being used to regenerate ribulose bisphosphate from triose phosphate. ADP and phosphate returning to the light-dependent reaction to be used in photophosphorylation. Exam tip: Pair the two requirements in one sentence: reduced NADP provides the hydrogen, hydrolysed ATP provides the energy.

    See it

    A flowchart running top to bottom. ATP splits into two branches. One branch leads to ADP and inorganic phosphate, labelled as produced by hydrolysis by ATP hydrolase. The other branch supplies energy to two steps: the conversion of glycerate 3-phosphate to triose phosphate, and the regeneration of ribulose bisphosphate from triose phosphate. From ADP and inorganic phosphate an arrow leads to photophosphorylation, which occurs on return to the thylakoid membranes, and from photophosphorylation an arrow leads back to ATP, closing the cycle.
    Shows ATP being hydrolysed to ADP and inorganic phosphate, its energy driving the reduction of GP and the regeneration of RuBP, and ADP and phosphate returning to photophosphorylation to remake ATP.

    Key words

    Say what each word means before you turn the card.

    ATP hydrolysis

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    energy for reduction

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    RuBP regeneration

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    ADP recycling

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    See every definition
    ATP hydrolysis
    ATP is hydrolysed to ADP and inorganic phosphate by ATP hydrolase, releasing energy in a small, immediately usable amount.
    energy for reduction
    The additional energy needed for the reduction of glycerate 3-phosphate comes from the hydrolysis of ATP made in the light-dependent reaction.
    RuBP regeneration
    The third ATP molecule used in each turn of the Calvin cycle is used in regenerating ribulose bisphosphate from triose phosphate, a step with an energy cost of its own.
    ADP recycling
    The ADP and phosphate produced return to the thylakoid membranes, where photophosphorylation converts them back to ATP.

    Label the diagram

    Some boxes have lost their names. Pick the right name for each number.

    The diagram again, with 2 boxes numbered instead of named. A flowchart running top to bottom. ATP splits into two branches. One branch leads to ADP and inorganic phosphate, labelled as produced by hydrolysis by ATP hydrolase. The other branch supplies energy to two steps: the conversion of glycerate 3-phosphate to triose phosphate, and the regeneration of ribulose bisphosphate from triose phosphate. From ADP and inorganic phosphate an arrow leads to photophosphorylation, which occurs on return to the thylakoid membranes, and from photophosphorylation an arrow leads back to ATP, closing the cycle.
    • 1Box 1

    • 2Box 2

    0 of 2 labelled

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