Biology A-Level
restriction endonucleases and Southern blotting
What restriction endonucleases and Southern blotting means in Biology A-Level, as defined for AQA.
Definition of restriction endonucleases and Southern blotting
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Reference: AQA
DNA is cut with restriction endonucleases, then fragments are transferred to a membrane by Southern blotting and detected with labelled DNA probes, producing a banding pattern.
Topics that use restriction endonucleases and Southern blotting
- Genetic fingerprinting (A-level only) (AQA A-Level)
Related terms
- DNA hybridisation
- gel electrophoresis
- PCR amplification
- preventing inbreeding
- variable number tandem repeats
- VNTR amplification
- forensic comparison
- genetic relationships
Using restriction endonucleases and Southern blotting in the exam
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