Biology A-Level
Thermal cycling stages
What Thermal cycling stages means in Biology A-Level, as defined for OCR.
Definition of Thermal cycling stages
Check what you remember
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Reference: OCR
Each cycle has three temperature stages: denaturation near 95 °C separates the strands, annealing around 55–65 °C lets primers bind, and extension near 72 °C lets polymerase add nucleotides 5′ to 3′.
Topics that use Thermal cycling stages
- Manipulating genomes (OCR A-Level)
Related terms
- Applications in analysing nucleic acids and proteins
- Applications in DNA analysis and quantification
- Chain-termination mechanism
- Charge-based migration in an electric field
- Comparison of scale, speed and cost
- Degeneracy of the code
- Evolutionary relationships between species
- Fluorescent detection and size separation
Using Thermal cycling stages in the exam
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