AQA · GCSE · Biology

    Course version: 8461 · Version from Sept 2018

    Required practical activity 9: measure the population size of a common…

    Full specification wording

    Levels of organisation: Required practical activity 9: measure the population size of a common species in a habitat. Use sampling techniques to investigate the effect of a factor on the distribution of this species.

    This practical has two linked aims: to estimate the population size of a common species and to investigate how a factor affects its distribution.

    Checked against the AQA 8461 specification (opens in a new tab) on .

    • Point 11 of 12
    • 4 key terms

    Study notes

    From the checked teaching notes for this specification point.

    The idea

    This practical has two linked aims: to estimate the population size of a common species and to investigate how a factor affects its distribution. A quadrat, usually a square frame, is placed at random positions in the habitat, for example by using random numbers as coordinates. The number of the chosen species inside each quadrat is recorded, and the mean per quadrat is calculated. Population size is then estimated by scaling the mean up to the whole area: estimated population = mean number per quadrat × total habitat area ÷ quadrat area. To study distribution, quadrats are placed along a transect line across the habitat, and the factor, such as light intensity or soil moisture, is measured at each point. Plotting species number against the factor shows whether distribution is affected.

    What earns the marks

    • Place quadrats at random positions, for example using random number coordinates, to avoid bias.
    • Count the chosen species in each quadrat and calculate a mean number per quadrat.
    • Estimate population size using mean number per quadrat × total habitat area ÷ quadrat area.
    • Use a transect to sample along a line and measure the chosen factor, such as light intensity, at each sample point.
    • Repeat samples and control variables such as quadrat size and sampling method to improve reliability.
    • Plot or compare species numbers against the factor to identify a relationship with distribution.

    Common mistakes and how to put them right

    • Choosing quadrat positions by eye where the species looks common.

      Put it right by using random numbers to select coordinates.

    • Forgetting to convert units so quadrat area and habitat area match.

      Put it right by converting both to the same unit, such as m², before calculating.

    • Changing quadrat size or counting method between samples.

      Put it right by keeping quadrat size and counting rules constant throughout.

    Exam tips

    • State clearly that random sampling reduces bias and that repeats improve reliability.
    • Show the population estimate calculation with units, for example mean per m² × total area in m².
    • When describing a transect, name the factor measured and the instrument used, such as a light meter for light intensity.

    Try the lesson

    The start of the Levels of organisation: Required practical activity 9: measure the population size of a common species in a habitat. Use sampling techniques to investigate the effect of a factor on the distribution of this species. lesson, made from checked teaching notes for this specification point.

    Have a go first

    A student uses a quadrat to sample the number of daisies in a field. What is the main reason for placing the quadrat at random?

    Listen

    This practical has two linked aims: to estimate the population size of a common species and to investigate how a factor affects its distribution. A quadrat, usually a square frame, is placed at random positions in the habitat, for example by using random numbers as coordinates. The number of the chosen species inside each quadrat is recorded, and the mean per quadrat is calculated. Population size is then estimated by scaling the mean up to the whole area: estimated population = mean number per quadrat × total habitat area ÷ quadrat area. To study distribution, quadrats are placed along a transect line across the habitat, and the factor, such as light intensity or soil moisture, is measured at each point. Plotting species number against the factor shows whether distribution is affected. A strong answer earns marks for these points. Place quadrats at random positions, for example using random number coordinates, to avoid bias. Count the chosen species in each quadrat and calculate a mean number per quadrat. Estimate population size using mean number per quadrat × total habitat area ÷ quadrat area. Use a transect to sample along a line and measure the chosen factor, such as light intensity, at each sample point. A common slip: Choosing quadrat positions by eye where the species looks common. Instead, using random numbers to select coordinates. Exam tip: State clearly that random sampling reduces bias and that repeats improve reliability.

    See it

    A top-to-bottom flowchart with two branches. The first branch starts with placing quadrats at random positions using random number coordinates, then counting the chosen species in each quadrat, then calculating the mean number per quadrat, then estimating population size as mean per quadrat times total habitat area divided by quadrat area. The second branch starts with placing quadrats along a transect line, then measuring the chosen factor at each sample point, then plotting species number against the factor, then identifying whether distribution is affected.
    Shows the two linked sampling routes: random quadrats to estimate population size, and a transect to investigate how a factor affects distribution.

    Key words

    Say what each word means before you turn the card.

    Random sampling to avoid bias

    Try answering aloud or in your head before turning the card.

    Sampling and estimation

    Try answering aloud or in your head before turning the card.

    Distribution along environmental gradients

    Try answering aloud or in your head before turning the card.

    Reliability, repeats and control variables

    Try answering aloud or in your head before turning the card.

    See every definition
    Random sampling to avoid bias
    Placing quadrats at random positions, for example using random number coordinates, so that bias is avoided.
    Sampling and estimation
    Counting the chosen species in each quadrat, calculating a mean number per quadrat, and scaling the mean up to the whole area to estimate population size.
    Distribution along environmental gradients
    Placing quadrats along a transect line across the habitat and measuring the chosen factor, such as light intensity or soil moisture, at each point to see whether distribution is affected.
    Reliability, repeats and control variables
    Repeating samples and keeping variables such as quadrat size and sampling method constant to improve reliability.

    Label the diagram

    Some boxes have lost their names. Pick the right name for each number.

    The diagram again, with 3 boxes numbered instead of named. A top-to-bottom flowchart with two branches. The first branch starts with placing quadrats at random positions using random number coordinates, then counting the chosen species in each quadrat, then calculating the mean number per quadrat, then estimating population size as mean per quadrat times total habitat area divided by quadrat area. The second branch starts with placing quadrats along a transect line, then measuring the chosen factor at each sample point, then plotting species number against the factor, then identifying whether distribution is affected.
    • 1Box 1

    • 2Box 2

    • 3Box 3

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