OCR · A-Level · Biology

    Course version: H420 · Version from Sept 2025

    Analysis: (ii) measurement of gradients and intercepts.

    A straight-line graph carries quantitative information in its slope and where it crosses an axis.

    Checked against the OCR H420 specification (opens in a new tab) on .

    • Point 4 of 4
    • 4 key terms

    Study notes

    From the checked teaching notes for this specification point.

    The idea

    A straight-line graph carries quantitative information in its slope and where it crosses an axis. To measure a gradient, choose two points on the line of best fit that are far apart (not two plotted data points, which may sit off the line), read their coordinates carefully using the axis scales, and calculate gradient = change in y ÷ change in x, giving units of y-unit per x-unit. A larger separation reduces reading error. To find an intercept, read the value where the line crosses the chosen axis: the y-intercept is the y-value at x = 0, and the x-intercept is the x-value at y = 0. If an axis does not start at zero, extend the line of best fit to the true zero position before reading the intercept, and state the intercept with its unit. Where the relationship is not linear, a tangent drawn at the required point allows a gradient to be estimated.

    What earns the marks

    • Defines gradient as the change in the y-variable divided by the change in the x-variable, taken from the line of best fit.
    • Explains that two widely separated points on the line should be used to reduce the effect of reading error.
    • States that the gradient carries units of the y-axis unit per x-axis unit.
    • Describes reading an intercept as the value where the line of best fit crosses the relevant axis, including its unit.
    • Recognises that if an axis does not begin at zero, the line must be extended to the true zero before the intercept is read.
    • Notes that a tangent at a point can be used to estimate the gradient of a curve.

    Common mistakes and how to put them right

    • Using two raw data points rather than points on the line of best fit.

      Put it right: Read coordinates from the drawn line so scatter is averaged out.

    • Dividing the change in x by the change in y.

      Put it right: Gradient is change in y divided by change in x.

    • Reading an intercept straight off a truncated axis without extending the line to zero.

      Put it right: Extrapolate the line of best fit to the true zero position first.

    Exam tips

    • Mark the two chosen points on the line clearly and show the coordinate values you read before calculating.
    • Carry the units through the gradient calculation and simplify them, for example cm³ min⁻¹.
    • When reading an intercept, check whether the axis starts at zero and extend the line if it does not.

    Try the lesson

    The start of the Analysis: (ii) measurement of gradients and intercepts. lesson, made from checked teaching notes for this specification point.

    Have a go first

    A student plots a graph of enzyme activity against substrate concentration. The line of best fit crosses the y-axis at 2.5 units. What does this intercept represent?

    Listen

    A straight-line graph carries quantitative information in its slope and where it crosses an axis. To measure a gradient, choose two points on the line of best fit that are far apart (not two plotted data points, which may sit off the line), read their coordinates carefully using the axis scales, and calculate gradient = change in y ÷ change in x, giving units of y-unit per x-unit. A larger separation reduces reading error. To find an intercept, read the value where the line crosses the chosen axis: the y-intercept is the y-value at x = 0, and the x-intercept is the x-value at y = 0. If an axis does not start at zero, extend the line of best fit to the true zero position before reading the intercept, and state the intercept with its unit. Where the relationship is not linear, a tangent drawn at the required point allows a gradient to be estimated. A strong answer earns marks for these points. Defines gradient as the change in the y-variable divided by the change in the x-variable, taken from the line of best fit. Explains that two widely separated points on the line should be used to reduce the effect of reading error. States that the gradient carries units of the y-axis unit per x-axis unit. Describes reading an intercept as the value where the line of best fit crosses the relevant axis, including its unit. A common slip: Using two raw data points rather than points on the line of best fit. Instead, read coordinates from the drawn line so scatter is averaged out. Exam tip: Mark the two chosen points on the line clearly and show the coordinate values you read before calculating.

    Key words

    Say what each word means before you turn the card.

    Gradient from a line of best fit

    Try answering aloud or in your head before turning the card.

    Choice of widely separated points

    Try answering aloud or in your head before turning the card.

    Intercepts and axis origin

    Try answering aloud or in your head before turning the card.

    Tangents for curved graphs

    Try answering aloud or in your head before turning the card.

    See every definition
    Gradient from a line of best fit
    The change in y divided by the change in x, taken from the line of best fit, giving units of y-unit per x-unit.
    Choice of widely separated points
    Two points on the line of best fit that are far apart, used because a larger separation reduces reading error.
    Intercepts and axis origin
    The value where the line of best fit crosses the chosen axis, read at the true zero position if the axis does not start at zero.
    Tangents for curved graphs
    A tangent drawn at the required point, which allows a gradient to be estimated where the relationship is not linear.

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