OCR · A-Level · Biology

    Course version: H420 · Version from Sept 2025

    (h) photomicrographs of cellular components in a range of eukaryotic cells

    Full specification wording

    Cell structure: (h) photomicrographs of cellular components in a range of eukaryotic cells

    Photomicrographs are images taken through a microscope, and interpreting them is a core practical skill.

    Checked against the OCR H420 specification (opens in a new tab) on .

    • Point 7 of 10
    • 4 key terms

    Study notes

    From the checked teaching notes for this specification point.

    The idea

    Photomicrographs are images taken through a microscope, and interpreting them is a core practical skill. You must recognise cellular components across a range of eukaryotic cells, not just one example. Learn to identify the nucleus (often large, with a darker nucleolus), mitochondria (oval with folded internal membranes), chloroplasts (green, stacked thylakoids), the cell surface membrane, a large permanent vacuole in plant cells, the cellulose cell wall, rough and smooth endoplasmic reticulum, Golgi apparatus and ribosomes. Use scale and magnification to judge real size, and compare animal, plant and fungal cells. Distinguish structures by shape, position, membrane pattern and staining, and note that resolution limits what can be seen.

    What earns the marks

    • Recognises the nucleus and nucleolus in a photomicrograph by its large size, boundary and darker internal region.
    • Identifies mitochondria by their oval profile and internal folded membranes (cristae) where resolution allows.
    • Identifies chloroplasts in plant cells by their green colour and internal stacked thylakoid membranes.
    • Distinguishes a plant cell from an animal cell using the cellulose cell wall and large permanent vacuole.
    • Uses the stated magnification or scale bar to estimate the actual size of a named organelle.
    • Recognises that some organelles (for example ribosomes) may not be resolvable at the stated magnification.

    Common mistakes and how to put them right

    • Assuming every round structure is a nucleus.

      Put it right: Is to check for a boundary and darker nucleolus and to compare with the scale.

    • Treating a photomicrograph of one cell type as representative of all eukaryotes.

      Put it right: Is to compare animal, plant and fungal examples.

    • Ignoring the scale bar and guessing size.

      Put it right: Is to measure the image and divide by magnification to give actual size.

    Exam tips

    • Read the magnification and scale bar before identifying any structure.
    • Match each structure to a named function to confirm your identification.
    • Eliminate options that describe structures not visible at the stated resolution.

    Try the lesson

    The start of the Cell structure: (h) photomicrographs of cellular components in a range of eukaryotic cells lesson, made from checked teaching notes for this specification point.

    Have a go first

    In a transmission electron micrograph, which feature identifies a mitochondrion?

    Listen

    Photomicrographs are images taken through a microscope, and interpreting them is a core practical skill. You must recognise cellular components across a range of eukaryotic cells, not just one example. Learn to identify the nucleus (often large, with a darker nucleolus), mitochondria (oval with folded internal membranes), chloroplasts (green, stacked thylakoids), the cell surface membrane, a large permanent vacuole in plant cells, the cellulose cell wall, rough and smooth endoplasmic reticulum, Golgi apparatus and ribosomes. Use scale and magnification to judge real size, and compare animal, plant and fungal cells. Distinguish structures by shape, position, membrane pattern and staining, and note that resolution limits what can be seen. A strong answer earns marks for these points. Recognises the nucleus and nucleolus in a photomicrograph by its large size, boundary and darker internal region. Identifies mitochondria by their oval profile and internal folded membranes (cristae) where resolution allows. Identifies chloroplasts in plant cells by their green colour and internal stacked thylakoid membranes. Distinguishes a plant cell from an animal cell using the cellulose cell wall and large permanent vacuole. A common slip: Assuming every round structure is a nucleus. Instead, is to check for a boundary and darker nucleolus and to compare with the scale. Exam tip: Read the magnification and scale bar before identifying any structure.

    See it

    A top-down flowchart. The first node, 'Read magnification and scale bar', leads to three branches. The first branch goes to 'Identify structures by shape, position, membrane pattern and staining', which then splits into five identification nodes: 'Nucleus: large, boundary, darker nucleolus'; 'Mitochondria: oval, folded internal membranes'; 'Chloroplasts: green, stacked thylakoids'; 'Cellulose cell wall and large permanent vacuole'; and 'Cell surface membrane, rough and smooth ER, Golgi apparatus'. The second branch from the first node goes to 'Estimate actual size from scale or magnification'. The third branch goes to 'Check resolution: some organelles, e.g. ribosomes, may not be resolvable'. All five identification nodes then lead to a final node, 'Compare animal, plant and fungal cells'.
    Shows how to interpret a photomicrograph by reading magnification and scale, identifying named cellular components, estimating actual size, checking resolution, and comparing animal, plant and fungal cells.

    Key words

    Say what each word means before you turn the card.

    Photomicrograph

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    Magnification and actual size

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    Resolution limits of microscopy

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    Eukaryotic cell diversity

    Try answering aloud or in your head before turning the card.

    See every definition
    Photomicrograph
    An image taken through a microscope, which you must interpret by recognising cellular components across a range of eukaryotic cells.
    Magnification and actual size
    Using the stated magnification or scale bar to estimate the actual size of a named organelle.
    Resolution limits of microscopy
    Resolution limits what can be seen, so some organelles may not be resolvable at the stated magnification.
    Eukaryotic cell diversity
    Recognising cellular components across a range of eukaryotic cells, not just one example, and comparing animal, plant and fungal cells.

    Label the diagram

    Some boxes have lost their names. Pick the right name for each number.

    The diagram again, with 3 boxes numbered instead of named. A top-down flowchart. The first node, 'Read magnification and scale bar', leads to three branches. The first branch goes to 'Identify structures by shape, position, membrane pattern and staining', which then splits into five identification nodes: 'Nucleus: large, boundary, darker nucleolus'; 'Mitochondria: oval, folded internal membranes'; 'Chloroplasts: green, stacked thylakoids'; 'Cellulose cell wall and large permanent vacuole'; and 'Cell surface membrane, rough and smooth ER, Golgi apparatus'. The second branch from the first node goes to 'Estimate actual size from scale or magnification'. The third branch goes to 'Check resolution: some organelles, e.g. ribosomes, may not be resolvable'. All five identification nodes then lead to a final node, 'Compare animal, plant and fungal cells'.
    • 1Box 1

    • 2Box 2

    • 3Box 3

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