AQA · GCSE · Combined Science

    Course version: 8464 · Version from Sept 2018

    Required practical activity 7: measure the population size of a common…

    Full specification wording

    Levels of organisation: Required practical activity 7: measure the population size of a common species in a habitat. Use sampling techniques to investigate the effect of a factor on the distribution of this species.

    This practical develops two linked skills.

    Checked against the AQA 8464 specification (opens in a new tab) on .

    • Point 11 of 11
    • 4 key terms

    Study notes

    From the checked teaching notes for this specification point.

    The idea

    This practical develops two linked skills. First, estimate population size of a common species by sampling, for example using a quadrat to count daisies in a field. Place quadrats randomly, such as by using a random number generator to select coordinates, count individuals in each quadrat, then scale up: estimated population = (total area ÷ quadrat area) × mean count per quadrat. Second, investigate how an abiotic factor, such as light intensity, affects distribution. Use a transect: run a tape across the habitat and place quadrats at regular intervals, recording the factor and the species count at each point. This shows whether the species becomes more or less abundant as the factor changes. Sampling avoids counting every organism, which is often impossible, but it must be random or systematic to reduce bias and be repeated to improve reliability.

    What earns the marks

    • Selects an appropriate sampling method: random quadrat placement for population size, or a transect for investigating distribution along an environmental gradient.
    • Uses a quadrat of known area and counts the species consistently, including a clear rule for organisms on the boundary.
    • Collects sufficient repeat samples and calculates a mean count per quadrat before scaling to the whole habitat area.
    • Measures the chosen factor, such as light intensity with a light meter, at each sampling point and records paired data.
    • Processes data appropriately, for example estimating population size = (total area ÷ quadrat area) × mean count, or plotting a graph of species number against the factor.
    • Evaluates limitations, such as quadrat size, sample number, random placement and time of day, and suggests improvements.

    Common mistakes and how to put them right

    • Placing quadrats only where the species is obvious.

      Put it right: Use random coordinates or a regular transect so every part of the habitat has a chance of being sampled.

    • Forgetting to convert units when scaling up, for example mixing cm² and m².

      Put it right: Convert all areas to the same unit before calculating estimated population size.

    • Recording only the species count and ignoring the factor being investigated.

      Put it right: Measure and record the factor at each quadrat so the effect on distribution can be analysed.

    Exam tips

    • State clearly whether you are estimating population size or investigating distribution, because the sampling method differs.
    • Show the scaling calculation with units, for example estimated population = (100 m² ÷ 0.25 m²) × 12 = 4800.
    • When describing improvements, link each one to the problem it reduces, such as more quadrats to reduce the effect of chance.

    Try the lesson

    The start of the Levels of organisation: Required practical activity 7: measure the population size of a common species in a habitat. Use sampling techniques to investigate the effect of a factor on the distribution of this species. lesson, made from checked teaching notes for this specification point.

    Have a go first

    In required practical activity 7, a student places a 0.25 m² quadrat on the ground and counts 12 daisies inside it. What is the population density of daisies in this quadrat?

    Listen

    This practical develops two linked skills. First, estimate population size of a common species by sampling, for example using a quadrat to count daisies in a field. Place quadrats randomly, such as by using a random number generator to select coordinates, count individuals in each quadrat, then scale up: estimated population = (total area ÷ quadrat area) × mean count per quadrat. Second, investigate how an abiotic factor, such as light intensity, affects distribution. Use a transect: run a tape across the habitat and place quadrats at regular intervals, recording the factor and the species count at each point. This shows whether the species becomes more or less abundant as the factor changes. Sampling avoids counting every organism, which is often impossible, but it must be random or systematic to reduce bias and be repeated to improve reliability. A strong answer earns marks for these points. Selects an appropriate sampling method: random quadrat placement for population size, or a transect for investigating distribution along an environmental gradient. Uses a quadrat of known area and counts the species consistently, including a clear rule for organisms on the boundary. Collects sufficient repeat samples and calculates a mean count per quadrat before scaling to the whole habitat area. Measures the chosen factor, such as light intensity with a light meter, at each sampling point and records paired data. A common slip: Placing quadrats only where the species is obvious: this biases results. Instead, use random coordinates or a regular transect so every part of the habitat has a chance of being sampled. Exam tip: State clearly whether you are estimating population size or investigating distribution, because the sampling method differs.

    See it

    A top-down flowchart. It starts with one node, 'Choose sampling method', which branches into two paths. The left path goes: 'Random quadrats for population size', then 'Place quadrats randomly', then 'Count species in each quadrat', then 'Calculate mean count per quadrat', then 'Estimate population = (total area ÷ quadrat area) × mean count'. The right path goes: 'Transect for effect of a factor on distribution', then 'Run tape across habitat', then 'Place quadrats at regular intervals', then 'Record factor and species count at each point', then 'Plot graph of species number against factor'.
    It shows the two sampling routes: random quadrats to estimate population size, and a transect to investigate how a factor affects distribution.

    Key words

    Say what each word means before you turn the card.

    Sampling and estimation

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    Random and systematic sampling

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    Abiotic factors and species distribution

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    Reliability, bias and data processing

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    See every definition
    Sampling and estimation
    Counting a common species in quadrats rather than every organism, then scaling the mean count up to the whole habitat area.
    Random and systematic sampling
    Placing quadrats randomly, such as by using a random number generator to select coordinates, or along a transect at regular intervals.
    Abiotic factors and species distribution
    Recording a factor such as light intensity at each sampling point to show whether the species becomes more or less abundant as the factor changes.
    Reliability, bias and data processing
    Repeating samples to improve reliability, sampling randomly or systematically to reduce bias, and calculating a mean count or plotting a graph.

    Label the diagram

    Some boxes have lost their names. Pick the right name for each number.

    The diagram again, with 3 boxes numbered instead of named. A top-down flowchart. It starts with one node, 'Choose sampling method', which branches into two paths. The left path goes: 'Random quadrats for population size', then 'Place quadrats randomly', then 'Count species in each quadrat', then 'Calculate mean count per quadrat', then 'Estimate population = (total area ÷ quadrat area) × mean count'. The right path goes: 'Transect for effect of a factor on distribution', then 'Run tape across habitat', then 'Place quadrats at regular intervals', then 'Record factor and species count at each point', then 'Plot graph of species number against factor'.
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