AQA · GCSE · Biology

    Course version: 8461 · Version from Sept 2018

    Tissue culture: using small groups of cells from part of a plant to grow…

    Full specification wording

    Cloning (biology only): Tissue culture: using small groups of cells from part of a plant to grow identical new plants. This is important for preserving rare plant species or commercially in nurseries.

    Tissue culture is a cloning method that uses small groups of cells taken from part of a plant, such as a shoot tip or leaf, to grow identical new plants.

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    • Point 1 of 6

    Study notes

    From the checked teaching notes for this specification point.

    The idea

    Tissue culture is a cloning method that uses small groups of cells taken from part of a plant, such as a shoot tip or leaf, to grow identical new plants. The cells are grown on a sterile nutrient medium containing hormones, and because they divide by mitosis the new plants are genetically identical clones of the parent. Sterile conditions prevent microorganisms contaminating the culture. Tissue culture is valuable for preserving rare plant species, because many identical plants can be produced from a small amount of material, and commercially in nurseries, where large numbers of uniform plants can be grown quickly.

    What earns the marks

    • Small groups of cells are taken from part of a plant, such as a shoot tip.
    • Cells are grown on a sterile nutrient medium with hormones.
    • The cells divide by mitosis to form identical new plants.
    • Tissue culture preserves rare plant species because many clones can be grown from limited material.
    • Commercially, nurseries use tissue culture to produce large numbers of uniform plants quickly.

    Common mistakes and how to put them right

    • Confusing tissue culture with taking cuttings.

      Put it right: Tissue culture uses small groups of cells grown on a medium, not detached stem pieces rooted in soil.

    • Saying the new plants are genetically different.

      Put it right: Mitosis produces genetically identical clones.

    • Forgetting sterile conditions; correction: contamination by microorganisms would destroy the culture.

    Exam tips

    • Name the source of cells and the growth medium in your answer.
    • State that mitosis makes the new plants identical clones.
    • Give one conservation reason and one commercial reason for using tissue culture.

    Try the lesson

    The start of the Cloning (biology only): Tissue culture: using small groups of cells from part of a plant to grow identical new plants. This is important for preserving rare plant species or commercially in nurseries. lesson, made from checked teaching notes for this specification point.

    Have a go first

    What is tissue culture used for in plants?

    Listen

    Tissue culture is a cloning method that uses small groups of cells taken from part of a plant, such as a shoot tip or leaf, to grow identical new plants. The cells are grown on a sterile nutrient medium containing hormones, and because they divide by mitosis the new plants are genetically identical clones of the parent. Sterile conditions prevent microorganisms contaminating the culture. Tissue culture is valuable for preserving rare plant species, because many identical plants can be produced from a small amount of material, and commercially in nurseries, where large numbers of uniform plants can be grown quickly. A strong answer earns marks for these points. Small groups of cells are taken from part of a plant, such as a shoot tip. Cells are grown on a sterile nutrient medium with hormones. The cells divide by mitosis to form identical new plants. Tissue culture preserves rare plant species because many clones can be grown from limited material. A common slip: Confusing tissue culture with taking cuttings. Instead, tissue culture uses small groups of cells grown on a medium, not detached stem pieces rooted in soil. Exam tip: Name the source of cells and the growth medium in your answer.

    See it

    A top-down flowchart. The first box says small groups of cells taken from part of a plant, and an arrow leads to a box saying cells grown on a sterile nutrient medium with hormones. An arrow leads to a box saying cells divide by mitosis, then to a box saying identical new plants (clones). From that box two arrows branch out: one to a box saying preserves rare plant species, and one to a box saying nurseries produce large numbers of uniform plants quickly.
    It shows how tissue culture grows identical new plants from small groups of cells and why this matters for conservation and nurseries.

    Label the diagram

    Some boxes have lost their names. Pick the right name for each number.

    The diagram again, with 3 boxes numbered instead of named. A top-down flowchart. The first box says small groups of cells taken from part of a plant, and an arrow leads to a box saying cells grown on a sterile nutrient medium with hormones. An arrow leads to a box saying cells divide by mitosis, then to a box saying identical new plants (clones). From that box two arrows branch out: one to a box saying preserves rare plant species, and one to a box saying nurseries produce large numbers of uniform plants quickly.
    • 1Box 1

    • 2Box 2

    • 3Box 3

    0 of 3 labelled

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