AQA · GCSE · Biology

    Course version: 8461 · Version from Sept 2018

    Students should be able to calculate cross-sectional areas of colonies or…

    Full specification wording

    Culturing microorganisms (biology only): Students should be able to calculate cross-sectional areas of colonies or clear areas around colonies using πr².

    A colony or a clear area around a colony is roughly circular, so its cross-sectional area is calculated using the formula for the area of a circle, A = πr².

    Checked against the AQA 8461 specification (opens in a new tab) on .

    • Point 6 of 11
    • 4 key terms

    Study notes

    From the checked teaching notes for this specification point.

    The idea

    A colony or a clear area around a colony is roughly circular, so its cross-sectional area is calculated using the formula for the area of a circle, A = πr². First measure the diameter of the colony or clear zone with a ruler, then halve it to find the radius. Substitute the radius into A = πr², using π = 3.14 or the calculator value, and give the area in mm² or cm². For example, a colony of diameter 10 mm has radius 5 mm, so area = π × 5² = 78.5 mm². If comparing clear areas around colonies, calculate each area separately and compare the values to judge which microorganism is more affected by an antibiotic.

    What earns the marks

    • Measure the diameter of the colony or clear area, then divide by 2 to obtain the radius.
    • Substitute the radius into the formula A = πr².
    • Use an appropriate value of π, such as 3.14, and carry out the calculation accurately.
    • Give the area with correct units, for example mm² or cm².
    • When comparing clear areas, calculate each area and use the values to support a conclusion about effectiveness.
    • Show the stages of the calculation so the method can be followed.

    Common mistakes and how to put them right

    • Using the diameter instead of the radius in A = πr².

      Put it right: Halve the diameter first.

    • Forgetting to square the radius.

      Put it right: Calculate r² before multiplying by π.

    • Giving the answer without units.

      Put it right: State the area in mm² or cm².

    Exam tips

    • Write down the measured diameter and the calculated radius before substituting into the formula.
    • Show each step of the calculation, including the value of π used.
    • Check that the final answer has squared units, such as mm².

    Try the lesson

    The start of the Culturing microorganisms (biology only): Students should be able to calculate cross-sectional areas of colonies or clear areas around colonies using πr². lesson, made from checked teaching notes for this specification point.

    Have a go first

    A circular bacterial colony has a radius of 3 mm. Using the formula πr², what is the cross-sectional area of the colony? Use π = 3.14.

    Listen

    A colony or a clear area around a colony is roughly circular, so its cross-sectional area is calculated using the formula for the area of a circle, A = πr². First measure the diameter of the colony or clear zone with a ruler, then halve it to find the radius. Substitute the radius into A = πr², using π = 3.14 or the calculator value, and give the area in mm² or cm². For example, a colony of diameter 10 mm has radius 5 mm, so area = π × 5² = 78.5 mm². If comparing clear areas around colonies, calculate each area separately and compare the values to judge which microorganism is more affected by an antibiotic. A strong answer earns marks for these points. Measure the diameter of the colony or clear area, then divide by 2 to obtain the radius. Substitute the radius into the formula A = πr². Use an appropriate value of π, such as 3.14, and carry out the calculation accurately. Give the area with correct units, for example mm² or cm². A common slip: Using the diameter instead of the radius in A = πr². Instead, halve the diameter first. Exam tip: Write down the measured diameter and the calculated radius before substituting into the formula.

    See it

    A top-to-bottom flowchart with seven steps. Step one: measure the diameter of the colony or clear area with a ruler. Step two: halve the diameter to find the radius. Step three: substitute the radius into A = πr². Step four: square the radius, then multiply by π (for example 3.14). Step five: give the area with correct units, such as mm² or cm². Step six: to compare clear areas, calculate each area separately. Step seven: compare the values to judge which microorganism is more affected by an antibiotic. Each step leads to the next.
    The sequence of steps for calculating the cross-sectional area of a colony or clear area using A = πr² and comparing clear areas.

    Put it in order

    Put these steps in the order they happen.

    1. 1. Compare the values to judge which microorganism is more affected by an antibiotic
    2. 2. To compare clear areas, calculate each area separately
    3. 3. Substitute the radius into A = πr²
    4. 4. Give the area with correct units, e.g. mm² or cm²
    5. 5. Measure the diameter of the colony or clear area with a ruler
    6. 6. Halve the diameter to find the radius
    7. 7. Square the radius, then multiply by π (e.g. 3.14)

    Key words

    Say what each word means before you turn the card.

    Circular area calculation

    Try answering aloud or in your head before turning the card.

    Measurement and units

    Try answering aloud or in your head before turning the card.

    Colony growth

    Try answering aloud or in your head before turning the card.

    Antibiotic clear zones

    Try answering aloud or in your head before turning the card.

    See every definition
    Circular area calculation
    A colony or a clear area around a colony is roughly circular, so its cross-sectional area is calculated using the formula for the area of a circle, A = πr².
    Measurement and units
    Measure the diameter with a ruler, halve it to find the radius, and give the area in mm² or cm².
    Colony growth
    The cross-sectional area of a colony is calculated using A = πr², with the radius found by halving the measured diameter.
    Antibiotic clear zones
    When comparing clear areas around colonies, calculate each area separately and compare the values to judge which microorganism is more affected by an antibiotic.

    Label the diagram

    Some boxes have lost their names. Pick the right name for each number.

    The diagram again, with 3 boxes numbered instead of named. A top-to-bottom flowchart with seven steps. Step one: measure the diameter of the colony or clear area with a ruler. Step two: halve the diameter to find the radius. Step three: substitute the radius into A = πr². Step four: square the radius, then multiply by π (for example 3.14). Step five: give the area with correct units, such as mm² or cm². Step six: to compare clear areas, calculate each area separately. Step seven: compare the values to judge which microorganism is more affected by an antibiotic. Each step leads to the next.
    • 1Box 1

    • 2Box 2

    • 3Box 3

    0 of 3 labelled

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