AQA · GCSE · Biology

    Course version: 8461 · Version from Sept 2018

    The fungus is grown on glucose syrup, in aerobic conditions, and the…

    Full specification wording

    Role of biotechnology: The fungus is grown on glucose syrup, in aerobic conditions, and the biomass is harvested and purified.

    To produce mycoprotein, Fusarium is grown in a fermenter supplied with glucose syrup as a nutrient and energy source.

    Checked against the AQA 8461 specification (opens in a new tab) on .

    • Point 4 of 6
    • 3 key terms

    Study notes

    From the checked teaching notes for this specification point.

    The idea

    To produce mycoprotein, Fusarium is grown in a fermenter supplied with glucose syrup as a nutrient and energy source. The culture is kept aerobic, meaning oxygen is supplied continuously, because the fungus respires aerobically to release energy for growth and reproduction. Temperature and pH are also controlled so that enzymes work at their optimum and the culture is not damaged. As the fungus grows, it forms a dense biomass. This biomass is then harvested from the fermenter, and purified so that it is safe and suitable to use as food. Purification removes excess water and unwanted materials, leaving the mycoprotein product. The skill is to sequence the process correctly and to explain why each condition is needed.

    What earns the marks

    • Glucose syrup provides a nutrient and energy source for the fungus.
    • Aerobic conditions mean oxygen is supplied so the fungus can respire aerobically.
    • Temperature and pH are controlled to maintain optimum enzyme activity and growth.
    • The fungal biomass is harvested from the fermenter after growth.
    • Purification makes the harvested biomass suitable for use as food.

    Common mistakes and how to put them right

    • Saying the fungus is grown anaerobically.

      Put it right: The process is aerobic, so oxygen must be supplied.

    • Thinking glucose syrup is the final food product.

      Put it right: Glucose syrup is the substrate, and the harvested fungal biomass is the product.

    • Omitting purification; correction: after harvesting, the biomass is purified before it is used as food.

    Exam tips

    • Write the stages in order: substrate supplied, aerobic growth, harvesting, purification.
    • Link aerobic conditions to respiration and energy release for growth.
    • Use the word optimum when explaining temperature and pH control.

    Try the lesson

    The start of the Role of biotechnology: The fungus is grown on glucose syrup, in aerobic conditions, and the biomass is harvested and purified. lesson, made from checked teaching notes for this specification point.

    Have a go first

    A fungus is grown in a large tank to make a useful product. The fungus is grown on glucose syrup with plenty of air supplied. What is the glucose syrup used for?

    Listen

    To produce mycoprotein, Fusarium is grown in a fermenter supplied with glucose syrup as a nutrient and energy source. The culture is kept aerobic, meaning oxygen is supplied continuously, because the fungus respires aerobically to release energy for growth and reproduction. Temperature and pH are also controlled so that enzymes work at their optimum and the culture is not damaged. As the fungus grows, it forms a dense biomass. This biomass is then harvested from the fermenter, and purified so that it is safe and suitable to use as food. Purification removes excess water and unwanted materials, leaving the mycoprotein product. The skill is to sequence the process correctly and to explain why each condition is needed. A strong answer earns marks for these points. Glucose syrup provides a nutrient and energy source for the fungus. Aerobic conditions mean oxygen is supplied so the fungus can respire aerobically. Temperature and pH are controlled to maintain optimum enzyme activity and growth. The fungal biomass is harvested from the fermenter after growth. A common slip: Saying the fungus is grown anaerobically. Instead, the process is aerobic, so oxygen must be supplied. Exam tip: Write the stages in order: substrate supplied, aerobic growth, harvesting, purification.

    See it

    A top-to-bottom flowchart with eight boxes linked by arrows. The first box says glucose syrup is supplied as a nutrient and energy source. An arrow leads to a box saying aerobic conditions, with oxygen supplied continuously. An arrow leads to a box saying temperature and pH are controlled. An arrow leads to a box saying the fungus respires aerobically to release energy for growth and reproduction. An arrow leads to a box saying a dense fungal biomass forms. An arrow leads to a box saying the biomass is harvested from the fermenter. An arrow leads to a box saying the biomass is purified. A final arrow leads to a box saying the mycoprotein product is suitable for use as food.
    It sequences the stages of mycoprotein production, from supplying glucose syrup and aerobic conditions through growth, harvesting and purification to the final product.

    Put it in order

    Put these steps in the order they happen.

    1. 1. Dense fungal biomass forms
    2. 2. Mycoprotein product suitable for use as food
    3. 3. Biomass harvested from the fermenter
    4. 4. Glucose syrup supplied as nutrient and energy source
    5. 5. Aerobic conditions: oxygen supplied continuously
    6. 6. Biomass purified
    7. 7. Temperature and pH controlled
    8. 8. Fungus respires aerobically to release energy for growth and reproduction

    Key words

    Say what each word means before you turn the card.

    Aerobic conditions

    Try answering aloud or in your head before turning the card.

    Glucose syrup

    Try answering aloud or in your head before turning the card.

    Biomass harvesting and purification

    Try answering aloud or in your head before turning the card.

    See every definition
    Aerobic conditions
    Oxygen is supplied continuously so the fungus can respire aerobically to release energy for growth and reproduction.
    Glucose syrup
    Supplied to the fungus as a nutrient and energy source.
    Biomass harvesting and purification
    The dense biomass is harvested from the fermenter and purified so that it is safe and suitable to use as food.

    Label the diagram

    Some boxes have lost their names. Pick the right name for each number.

    The diagram again, with 3 boxes numbered instead of named. A top-to-bottom flowchart with eight boxes linked by arrows. The first box says glucose syrup is supplied as a nutrient and energy source. An arrow leads to a box saying aerobic conditions, with oxygen supplied continuously. An arrow leads to a box saying temperature and pH are controlled. An arrow leads to a box saying the fungus respires aerobically to release energy for growth and reproduction. An arrow leads to a box saying a dense fungal biomass forms. An arrow leads to a box saying the biomass is harvested from the fermenter. An arrow leads to a box saying the biomass is purified. A final arrow leads to a box saying the mycoprotein product is suitable for use as food.
    • 1Box 1

    • 2Box 2

    • 3Box 3

    0 of 3 labelled

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